Volume 21, Issue 4 (4-2025)
lmj 2025, 21(4): 22-27 |
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Gholami S, Habibi M, Kouhkan A, Partoazar A, Nasiri N. The Effect of Combined Injection of Bone Marrow-Derived Mesenchymal Stem Cells and Autologous Dermal Fibroblasts on Collagen Synthesis in a Diabetic Mouse Model of Skin Wound Healing. lmj 2025; 21 (4) :22-27
URL: http://icml.ir/article-1-665-en.html
URL: http://icml.ir/article-1-665-en.html
Abstract: (510 Views)
Background: Diabetes is a significant factor in delayed wound healing, often associated with impaired collagen synthesis. This study aimed to investigate the effect of simultaneous injection of bone marrow-derived mesenchymal stem cells (BM-MSCs) and autologous dermal fibroblasts on collagen synthesis and deposition in a diabetic mouse model of skin wounds.
Materials and Methods: In this experimental study, diabetic mice were divided into four groups: a group receiving BM-MSCs, a group receiving fibroblasts, a group receiving simultaneous injection of BM-MSCs and fibroblasts, a control (Untreated group). Collagen synthesis and deposition were evaluated using Masson's trichrome staining.
Results: The results showed that the group receiving simultaneous injection of BM-MSCs and fibroblasts exhibited the highest levels of collagen synthesis and deposition. This was followed by the group receiving BM-MSCs alone, then the group receiving fibroblasts alone, and finally, the control group, which showed the lowest levels of collagen.
Conclusion: The simultaneous injection of BM-MSCs and autologous dermal fibroblasts can be considered an effective approach for improving skin wound healing in diabetic patients.
Materials and Methods: In this experimental study, diabetic mice were divided into four groups: a group receiving BM-MSCs, a group receiving fibroblasts, a group receiving simultaneous injection of BM-MSCs and fibroblasts, a control (Untreated group). Collagen synthesis and deposition were evaluated using Masson's trichrome staining.
Results: The results showed that the group receiving simultaneous injection of BM-MSCs and fibroblasts exhibited the highest levels of collagen synthesis and deposition. This was followed by the group receiving BM-MSCs alone, then the group receiving fibroblasts alone, and finally, the control group, which showed the lowest levels of collagen.
Conclusion: The simultaneous injection of BM-MSCs and autologous dermal fibroblasts can be considered an effective approach for improving skin wound healing in diabetic patients.
Keywords: Regeneration, Chronic wound, Mesenchymal stem cell, Autologous dermal fibroblast, Collagen
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Received: 2025/05/4 | Accepted: 2025/04/30 | Published: 2025/06/30
Received: 2025/05/4 | Accepted: 2025/04/30 | Published: 2025/06/30
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