Volume 21, Issue 4 (4-2025)
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Hadizadeh M. Effect of Radachlorin-mediated photodynamic therapy on breast cancer cells (MDA-MB-231). lmj 2025; 21 (4)
URL: http://icml.ir/article-1-662-en.html
URL: http://icml.ir/article-1-662-en.html
Abstract: (66 Views)
Objectives: Photodynamic therapy is an effective approach for cancer treatment that combines light with photosensitizing agents to produce free radicals and damage cancerous tissue. This research investigates the effects of Radachlorin as a photosensitizer in photodynamic therapy on MDA-MB-231 human breast cancer cell line, specifically exploring the induction of apoptosis in these cells.
Methods & Materials: In this experimental study, human breast cancer cells (MDA-MB-231 cells) were treated with Radachlorin for 4 hours and subsequently exposed to red-light laser (662 nm), at two energy densities (10 and 20 J/cm2). The cell viability was measured using the MTT assay. The mechanism of cell death was determined by flow cytometry.
Results: The results showed that radaclorin alone had little cytotoxicity. However, following red-light illumination, it had a strong cytotoxic effect on MDA-MB-231 cells. Light (20 J/cm2) and Radachlorin (100 µg/mL) decreased the percent of cell survival to 31%. The values of Radachlorin IC50 on MDA-MB-231 cells were 73 µg/ml and 54 µg/ml, 24 h after exposure to 10 J/cm2 and 20 J/cm2, respectively. Therefore, the effect of Radachlorin/PDT on MDA-MB-231 cells depended on the concentration of photosensitizer and the light dose used. We also observed induction of apoptosis in MDA-MB-231 cells following Radachlorin-mediated photodynamic therapy.
Conclusions: These data suggest that Radachlorin-mediated photodynamic therapy can be an effective therapeutic option for the management of breast cancer. However, more in vitro and in vivo studies are needed on the safety and efficacy of this treatment.
Methods & Materials: In this experimental study, human breast cancer cells (MDA-MB-231 cells) were treated with Radachlorin for 4 hours and subsequently exposed to red-light laser (662 nm), at two energy densities (10 and 20 J/cm2). The cell viability was measured using the MTT assay. The mechanism of cell death was determined by flow cytometry.
Results: The results showed that radaclorin alone had little cytotoxicity. However, following red-light illumination, it had a strong cytotoxic effect on MDA-MB-231 cells. Light (20 J/cm2) and Radachlorin (100 µg/mL) decreased the percent of cell survival to 31%. The values of Radachlorin IC50 on MDA-MB-231 cells were 73 µg/ml and 54 µg/ml, 24 h after exposure to 10 J/cm2 and 20 J/cm2, respectively. Therefore, the effect of Radachlorin/PDT on MDA-MB-231 cells depended on the concentration of photosensitizer and the light dose used. We also observed induction of apoptosis in MDA-MB-231 cells following Radachlorin-mediated photodynamic therapy.
Conclusions: These data suggest that Radachlorin-mediated photodynamic therapy can be an effective therapeutic option for the management of breast cancer. However, more in vitro and in vivo studies are needed on the safety and efficacy of this treatment.
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