Volume 20, Issue 2 (Lasers in Medicine 2023)
lmj 2023, 20(2): 22-30 |
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Bakhtiari M, NaimAbadi A, Shakeri F. Investigating the viability of glioblastoma cancer cells by modifying the parameters of cold atmospheric pressure plasma jet. lmj 2023; 20 (2) :22-30
URL: http://icml.ir/article-1-620-en.html
URL: http://icml.ir/article-1-620-en.html
Abstract: (733 Views)
Background and purpose: Brain tumors are the consequence of abnormal growth of cells, which may be benign or malignant depending on the nature of the constituent cells. Glioblastoma multiforme (GBM) is one of the most prevalent types of central nervous system tumors. Due to the resistance of glioblastoma tumor to chemotherapy and radiotherapy and the impracticality of complete tumor removal because of its aggressive nature, finding a novel approach is always of great interest. Studies conducted under laboratory conditions indicate that cold plasma destroys cancer cells without harming normal cells. Owing to the complex nature of plasma, its biological effects rest on the characteristics of the source and the type of target being treated. Therefore, in this study, two basic issues including the optimization of plasma jet voltage, time and distance for the treatment of glioblastoma cancer cell line and the effect of a mixture of 5% molecular oxygen gas with helium and argon working gases on the viability of these cells were investigated.
Materials and methods: Human glioblastoma cancer cell line U-87 MG was purchased from the cell bank of Pasteur Institute of Iran. For the optimization to occur, five working voltages, three plasma nozzle-to-cell distances and a treatment duration were considered, and the viability of the treated cells was measured by MTT test and based on the manufacturer's protocol. After configuring the experiment under optimal conditions, five treatment durations were investigated. In the experiment to investigate the effect of plasma jet working gas on the viability of cancer cells, 5% oxygen gas was added to helium or argon working gas and the results were compared. All statistical analyses were performed using SPSS and Statistica softwares.
Results: The results of voltage and distance optimization demonstrated that the working voltage of 4.5 kV and 4 cm distance proves to be more effective on the programmed death of U-87 MG cells for the selected plasma jet configuration. In addition, the results showed the inverse dependence of treatment time and survival of U-87 MG cells. The combination of 5% oxygen molecular gas with helium and argon led to an increase in survival rate and as a result, a decrease in plasma effectiveness.
Conclusion: In general, the present study indicated that different parameters of plasma jet, including voltage, duration of treatment, distance and type of working gas have an effect on the viability of U-87 MG cells, and the optimal treatment conditions for each cell line are specific according to the type of the device.
Materials and methods: Human glioblastoma cancer cell line U-87 MG was purchased from the cell bank of Pasteur Institute of Iran. For the optimization to occur, five working voltages, three plasma nozzle-to-cell distances and a treatment duration were considered, and the viability of the treated cells was measured by MTT test and based on the manufacturer's protocol. After configuring the experiment under optimal conditions, five treatment durations were investigated. In the experiment to investigate the effect of plasma jet working gas on the viability of cancer cells, 5% oxygen gas was added to helium or argon working gas and the results were compared. All statistical analyses were performed using SPSS and Statistica softwares.
Results: The results of voltage and distance optimization demonstrated that the working voltage of 4.5 kV and 4 cm distance proves to be more effective on the programmed death of U-87 MG cells for the selected plasma jet configuration. In addition, the results showed the inverse dependence of treatment time and survival of U-87 MG cells. The combination of 5% oxygen molecular gas with helium and argon led to an increase in survival rate and as a result, a decrease in plasma effectiveness.
Conclusion: In general, the present study indicated that different parameters of plasma jet, including voltage, duration of treatment, distance and type of working gas have an effect on the viability of U-87 MG cells, and the optimal treatment conditions for each cell line are specific according to the type of the device.
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