Volume 19, Issue 4 (4-2023)
lmj 2023, 19(4): 1-8 |
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Allahyari D, keshmniri Neghab H, Rezakhani N. Effectiveness Photobiomodulation therapy on reproduction, colonization and immigration of fibroblast cells in normal and diabetic conditions. lmj 2023; 19 (4) :1-8
URL: http://icml.ir/article-1-579-en.html
URL: http://icml.ir/article-1-579-en.html
Department of Biochemistry and Biophysics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Abstract: (1091 Views)
Purpose: One of the most common chronic diabetic complications is related on foot. The important effects of wounds on physical and mental health guide the search for effective treatments. One of the treatment methods that has been developed in recent years is low Low-level laser.
Low-level laser therapy (LLLT) is a medical procedure used to treat pain and accelerate wound healing. Low level laser changes the healing process at the cellular level and can increases releasing the growth factors from fibroblasts, so causes cell proliferation. In this research, the effects of low-laser radiation was investigated on wound healing process in normal and diabetic human fibroblast cell lines L929. Studies can show that low level laser in the appropriate dose and time can increase the wound healing process and that mean can be a treatment for diabetic wounds.
Methods: An interventional and in vitro study was carried out in the cell culture laboratory of Tehran Medical Sciences Academic Organization in 1400-1401.At first, human skin fibroblast cells L929 cultured in DMEM growth medium. A group of cells became diabetic with 17 micro molar concentration of glucose for 24 hour duration. After treatment the diabetic and normal cells exposed 3 J/cm2 dose of low-level laser in 3 continuous days, once a day for 3 minutes .investigated cell viability and cell migration assay with MTT test, colony assay test and inverted light microscope methods.
Results: The result of diabetic and normal treatment with 3 J/cm2 dose of low-level laser, shows that viability of cell and colonies number has been increased and the cells which didn’t any radiation had the least viability in wound healing process.
Discussion: Generally it can be said that, low-level laser radiation could change cell proliferation process and used appropriate dose and time can be effective for diabetic wound treatment.
Low-level laser therapy (LLLT) is a medical procedure used to treat pain and accelerate wound healing. Low level laser changes the healing process at the cellular level and can increases releasing the growth factors from fibroblasts, so causes cell proliferation. In this research, the effects of low-laser radiation was investigated on wound healing process in normal and diabetic human fibroblast cell lines L929. Studies can show that low level laser in the appropriate dose and time can increase the wound healing process and that mean can be a treatment for diabetic wounds.
Methods: An interventional and in vitro study was carried out in the cell culture laboratory of Tehran Medical Sciences Academic Organization in 1400-1401.At first, human skin fibroblast cells L929 cultured in DMEM growth medium. A group of cells became diabetic with 17 micro molar concentration of glucose for 24 hour duration. After treatment the diabetic and normal cells exposed 3 J/cm2 dose of low-level laser in 3 continuous days, once a day for 3 minutes .investigated cell viability and cell migration assay with MTT test, colony assay test and inverted light microscope methods.
Results: The result of diabetic and normal treatment with 3 J/cm2 dose of low-level laser, shows that viability of cell and colonies number has been increased and the cells which didn’t any radiation had the least viability in wound healing process.
Discussion: Generally it can be said that, low-level laser radiation could change cell proliferation process and used appropriate dose and time can be effective for diabetic wound treatment.
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Received: 2023/04/29 | Accepted: 2023/04/30 | Published: 2023/04/30
Received: 2023/04/29 | Accepted: 2023/04/30 | Published: 2023/04/30
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