Volume 18, Issue 4 (Journaloflasersinmedicine 2022)
lmj 2022, 18(4): 56-56 |
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Nategh-Ahmadi M H, Safaei A, Kafi M, Nadgaran H. Raman spectroscopy-based method to examine the oocyte quality. lmj 2022; 18 (4) :56-56
URL: http://icml.ir/article-1-553-en.html
URL: http://icml.ir/article-1-553-en.html
Animal Reproduction, Dept. of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran The corresponding Author, kafi@shirazu.ac.ir
Abstract: (2014 Views)
The medical and veterinary sciences researchers have been interested to develop and use non-invasive and non-destructive methods to evaluate the oocytes. Among oocyte quality evaluation methods, Raman spectroscopy technology has created promising horizons to meet this growing necessity. This method provides valuable and applied information about the oocyte quality based on the biochemical compositions and its alterations through irradiating and collecting back the laser beam. Qualitative evaluation on the bases of quantitative data analysis is provided to the embryologist, by using this technology. By using robust statistical analysis, Raman spectroscopy can accurately assess the biochemical differences between oocytes with different quality or stages of development. However, there are some challenges in assessing the quality of the oocytes such as the technology infancy, lack of clarity of its all angels and possible effects of laser beam on the cell. On the other hand, ahead horizons in evaluating oocyte quality by using this method are promising. In the current paper, we introduce biophysical principles and discuss the application of Raman spectroscopy for evaluation of the mammalian oocyte quality. This technique was used for evaluation of some biochemical compositions of the bovine in vitro matured oocyte in the present our preliminary research work.
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