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Rezakhani N, Soheilifar M H, Keshmiri Neghab H. Effects of Mesenchymal Stem Cell-Conditioned Medium on Human Dermal Fibroblasts and Epidermoid Carcinoma Cells: Implications for Wound Healing. ASWTR 2025; 22 (4) :8-14
URL: http://icml.ir/article-1-689-fa.html

Background: This investigation was directed to studying the impact of mesenchymal stem cells (MSCs) derived- conditioned medium (CM) in normal human dermal fibroblasts (HDF-a), and A431 epidermal carcinoma.
Methods: The cancerous and normal cell lines (A431 and HDF-a) were treated with four groups (DMEM, DMEM + FBS, CM, and CM + FBS) for 24, 48, and 72 hours. Then, the rate of proliferation and migration and TGF-β gene expression were examined using MTT assay, scratch assay, and real-time PCR respectively.
Results: Data showed that the MSCs-conditioned medium increased proliferation of HDF-a, whereas it had no significant effect on A431. Migration rate was faster in HDF-a than in A431 in scratch assay. The expression of TGF-β was up-regulated in HDF-a cell treated with conditioned media compared to the skin cancer cells.
Conclusion: This work suggests that MSCs derived- conditioned medium may play a role in skin wound healing by increasing proliferation and migration, along with alleviating TGF-β. Our findings show the interaction of A431 epidermoid carcinoma cells with MSC-conditioned medium is facing controversial concerns.