Volume 21, Issue 1 (7-2024)
lmj 2024, 21(1): 15-22 |
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ilchi M, nezameslami S, sajjad B, montazerghaem M. The effect of photodynamic treatment of photosensitizing drug
ALA-5 on the viability of glioblastoma cancer cells (6C). lmj 2024; 21 (1) :15-22
URL: http://icml.ir/article-1-634-en.html
URL: http://icml.ir/article-1-634-en.html
Abstract: (646 Views)
Goals: Glioblastoma is one of the types of brain tumors with the highest degree of malignancy and high recurrence. Current treatment methods include surgery, radiation therapy, and chemotherapy, each of which has many side effects. Photodynamic therapy (PDT), a new method of inducing cell death based on the interaction of three photosensitive drug agents, light source and oxygen, is selective and minimally invasive and has fewer side effects than conventional methods. In this method, the combination of light-sensitive drug and laser beam through photochemical interactions causes the production of active toxic oxygen (cytotoxic) and free radicals that selectively cause cell death. The aim of this research is to investigate the effect of PDT on the survival of glioblastoma cell line (6C) using the photosensitizer ALA-5 in different concentrations.
analysis method: First, 6C cells were cultured in DMEM medium. Then, the treatment of cancer cells by a low-power continuous diode laser at a wavelength of 635 nm with a power of 20 mW, at optical doses of 56.4 J/cm and 112.8 J/cm and different drug concentrations (25, 50, 75, 100 and μg 125/ml) has been checked by MTT test method.
findings: The results show that in the PDT method of 6C cells with ALA-5 photosensitizer, there is a significant decrease in the number of cells in the photodynamic therapy group compared to the ALA-5 group without laser and the laser without ALA-5. The application of ALA-5 drug increases the production of the main light-sensitive substance PPIX and as a result increases cell death.
Conclusion: Photodynamic treatment of 6C cancer cells with ALA-5 drug at a concentration of 75 μg/ml and an incubation time of 4 h along with diode laser radiation at a wavelength of 635 nm with a light dose of 112.8 2J/cm is optimal and the most effective in the death of cancer cells. It comes with in this case, the biological power of the cells decreases by 37%. The results show the sensitivity of malignant glioblastoma cancer cells to this treatment method
analysis method: First, 6C cells were cultured in DMEM medium. Then, the treatment of cancer cells by a low-power continuous diode laser at a wavelength of 635 nm with a power of 20 mW, at optical doses of 56.4 J/cm and 112.8 J/cm and different drug concentrations (25, 50, 75, 100 and μg 125/ml) has been checked by MTT test method.
findings: The results show that in the PDT method of 6C cells with ALA-5 photosensitizer, there is a significant decrease in the number of cells in the photodynamic therapy group compared to the ALA-5 group without laser and the laser without ALA-5. The application of ALA-5 drug increases the production of the main light-sensitive substance PPIX and as a result increases cell death.
Conclusion: Photodynamic treatment of 6C cancer cells with ALA-5 drug at a concentration of 75 μg/ml and an incubation time of 4 h along with diode laser radiation at a wavelength of 635 nm with a light dose of 112.8 2J/cm is optimal and the most effective in the death of cancer cells. It comes with in this case, the biological power of the cells decreases by 37%. The results show the sensitivity of malignant glioblastoma cancer cells to this treatment method
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