en عمومى General پژوهشي Research <div style="text-align: justify;"><strong>Background:</strong> In recent years, the number of recombinant proteins used for therapeutic applications has increased dramatically. Among the growth factors, platelet-derived growth factors (PDGFs) are effective in the management of injury and involve many cellular events in the healing process, including inflammatory cell recruitment, fibroblast proliferation and migration, collagen deposition, and granulation tissue formation. During the process of natural wound healing, platelets are one of the first cells that respond at the wound site or around it and play an essential role in wound healing. Platelets are a rich source of growth factors, including PDGF. PDGF is the first and only recombinant growth factor approved by the US Food and Drug Administration (FDA) for topical administration to treat diabetic foot ulcers.<br> <strong>Methods:</strong> In this study, we&rsquo;ve built a microbial expression vector containing the coding sequence for optimized PDGF-BB and transformed it into an E. coli Strain. Then we developed a complete production process that includes: expression, purification using affinity chromatography, and biological activity assay by evaluation of NIH-3T3 cell growth in response to the produced recombinant human PDGF-BB.<br> <strong>Results: </strong>The purified PDGF-BB (15 mg&frasl;L of culture) was well-folded and biologically active. The whole procedure is fast and scalable.<br> <strong>Conclusion:</strong> The chosen E. coli Strain can produce and fold recombinant human PDGF-BB. The produced PDGF-BB has biologically active, and could be considered for further investigation.</div> Recombinant human PDGF-BB, Recombinant DNA Technology, Microbial Expression System, Wound Healing 41 47 http://icml.ir/browse.php?a_code=A-10-582-1&slc_lang=en&sid=1 Armin Nazemi Zadeh nazemizadeh@acecr.ac.ir 1050031947532846005583 1050031947532846005583 Yes Department of Regenerative Medicine in Wound Healing, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran Fereshteh Sarafrazi Sarafrazif@yahoo.com 1050031947532846005584 1050031947532846005584 No Department of Regenerative Medicine in Wound Healing, Medical Laser Research Center, Yara Institute Academic Center for Education, Culture and Research (ACECR), Tehran, Iran Masoud Habibi Fozon Abadi m.habibi@acercr.ac.ir 1050031947532846005585 1050031947532846005585 No Department of Regenerative Medicine in Wound Healing, Medical Laser Research Center, Yara Institute Academic Center for Education, Culture and Research (ACECR), Tehran, Iran Hassan Rassouli rassouli@acecr.ac.ir 1050031947532846005586 0009-0002-8383-5275 No Department of Regenerative Medicine in Wound Healing, Medical Laser Research Center, Yara Institute Academic Center for Education, Culture and Research (ACECR), Tehran, Iran