Volume 19, Issue 1 (Journaloflasersinmedicine 2022)
lmj 2022, 19(1): 33-43 |
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Marashipour R, Shirmardi A. Coating Of Silver Nanoparticles by Sputtering Method on Glass Substrates as Surface-Enhanced Raman Spectroscopy (SERS) Biosensor for Detection of Whey Protein. lmj 2022; 19 (1) :33-43
URL: http://icml.ir/article-1-561-en.html
URL: http://icml.ir/article-1-561-en.html
Department of Chemistry, Faculty of Basic Sciences, Masjed Soleiman Azad University, 06143260093, Masjed Soleiman, Iran
Abstract: (863 Views)
Introduction: Whey protein is one of the most widely used colloidal proteins in the food, pharmaceutical, and medical industries and is also a popular dietary supplement to improve muscle strength and prevent various heart diseases, diabetes and osteoporosis. In addition, Surface-Enhanced Raman Scattering (SERS) is one of the most sensitive analytical tools presently known. In some cases, it is possible to record a high-quality SERS spectrum in which even a single molecule is involved. In this paper, plasmonic silver substrates that have increased the Raman signal have been used to identify whey protein.
Methods: In this experimental study, in order to make a SERS biosensor, silver nanoparticles were coated on the glass by the sputtering method, and the whey protein was detected using Raman spectroscopy as a non-destructive technique.
Results: The formation of silver nanoparticles was confirmed by observing the FCC structure in its XRD characterization. The plasmonic peak of the extinction spectrum of silver plasmonic substrates was observed around 439 nm. Moreover, the Field Emission Scanning Electron Microscope (FE-SEM) images of silver plasmonic substrates show that a large number of silver particles are between 20 and 40 nm in size. The roughness of the plasmonic substrates resulted from the non-uniformity of the silver coating leads to light scattering from the spots observed in the electron microscope image. The roughness observed for plasmonic substrates in the Atomic Force Microscope (AFM) image contributes to the scattering of light from the rough spots. By depositing the whey protein molecule on plasmonic substrates, its molecular vibrations were detected. In the following, the calibration chart of different concentrations of whey protein was measured.
Conclusion: Raman spectroscopy is an important method to identify molecules and is extensively used in determining the chemical and structural characteristics of various substances. Many materials have special Raman spectra; therefore, this phenomenon has turned the Raman device into an efficient tool for studying molecules' structural and chemical characteristics. By employing these plasmonic silver substrates and the SERS phenomenon, rapid and straightforward detection of whey protein can be performed up to a concentration of 10-7 M. Meanwhile, the concentration of whey protein can be obtained by calibration, using silver plasmonic substrates, and employing Raman spectroscopy.
Methods: In this experimental study, in order to make a SERS biosensor, silver nanoparticles were coated on the glass by the sputtering method, and the whey protein was detected using Raman spectroscopy as a non-destructive technique.
Results: The formation of silver nanoparticles was confirmed by observing the FCC structure in its XRD characterization. The plasmonic peak of the extinction spectrum of silver plasmonic substrates was observed around 439 nm. Moreover, the Field Emission Scanning Electron Microscope (FE-SEM) images of silver plasmonic substrates show that a large number of silver particles are between 20 and 40 nm in size. The roughness of the plasmonic substrates resulted from the non-uniformity of the silver coating leads to light scattering from the spots observed in the electron microscope image. The roughness observed for plasmonic substrates in the Atomic Force Microscope (AFM) image contributes to the scattering of light from the rough spots. By depositing the whey protein molecule on plasmonic substrates, its molecular vibrations were detected. In the following, the calibration chart of different concentrations of whey protein was measured.
Conclusion: Raman spectroscopy is an important method to identify molecules and is extensively used in determining the chemical and structural characteristics of various substances. Many materials have special Raman spectra; therefore, this phenomenon has turned the Raman device into an efficient tool for studying molecules' structural and chemical characteristics. By employing these plasmonic silver substrates and the SERS phenomenon, rapid and straightforward detection of whey protein can be performed up to a concentration of 10-7 M. Meanwhile, the concentration of whey protein can be obtained by calibration, using silver plasmonic substrates, and employing Raman spectroscopy.
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Received: 2022/10/15 | Accepted: 2022/10/2 | Published: 2022/10/2
Received: 2022/10/15 | Accepted: 2022/10/2 | Published: 2022/10/2
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