en عمومى General پژوهشي Research <div style="text-align: justify;"><strong>Background:</strong> This investigation was directed to studying the impact of mesenchymal stem cells (MSCs) derived- conditioned medium (CM) in normal human dermal fibroblasts (HDF-a), and A431 epidermal carcinoma.<br> <strong>Methods:</strong> The cancerous and normal cell lines (A431 and HDF-a) were treated with four groups (DMEM, DMEM + FBS, CM, and CM + FBS) for 24, 48, and 72 hours. Then, the rate of proliferation and migration and TGF-&beta; gene expression were examined using MTT assay, scratch assay, and real-time PCR respectively.<br> <strong>Results:</strong> Data showed that the MSCs-conditioned medium increased proliferation of HDF-a, whereas it had no significant effect on A431. Migration rate was faster in HDF-a than in A431 in scratch assay. The expression of TGF-&beta; was up-regulated in HDF-a cell treated with conditioned media compared to the skin cancer cells.<br> <strong>Conclusion:</strong> This work suggests that MSCs derived- conditioned medium may play a role in skin wound healing by increasing proliferation and migration, along with alleviating TGF-&beta;. Our findings show the interaction of A431 epidermoid carcinoma cells with MSC-conditioned medium is facing controversial concerns.</div> Conditioned medium, Mesenchymal Stem Cells (MSCs), Human Dermal Fibroblast, A431 Epidermal Carcinoma 8 14 http://icml.ir/browse.php?a_code=A-10-221-3&slc_lang=en&sid=1 Nakisa Rezakhani rezakhaninakisa@gmail.com 1050031947532846005630 1050031947532846005630 No Department of Biochemistry and Biophysics, TeMS.C., Islamic Azad University, Tehran, Iran Mohammad Hasan Soheilifar soheilih@gmail.com 1050031947532846005629 1050031947532846005629 No Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran Hoda Keshmiri Neghab hodakeshmiri@ut.ac.ir 1050031947532846005628 1050031947532846005628 Yes Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran