fa بررسی زنده‌مانی سلول‌های سرطان گلیوبلاستوما با تغییر پارامترهای جت پلاسمای فشار اتمسفری عمومى General پژوهشي Research <div style="text-align: justify;"><span style="line-height:2;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="direction:rtl"><span style="unicode-bidi:embed"><span class="StyleLatinHelveticaComplex2LotusLatin12ptComple" new="" roman="" times=""><span style="color:black"><b><span lang="AR-SA"><span style="color:red">اهداف:</span></span></b></span></span><span lang="AR-SA"> تومورهای مغز حاصل رشد غیرعادی سلول&nbsp;</span><span lang="AR-SA">هاست که بنا به ماهیت سلول‌های تشکیل</span><span lang="FA">&nbsp;</span><span lang="AR-SA">دهنده، ممکن است خوش خ</span><span lang="AR-SA">یم یا بدخیم باشند. گلیوبلاستومای چند</span><span lang="FA">&lrm;</span><span lang="AR-SA">شکلی (</span><span dir="LTR">GBM</span><span lang="AR-SA">) یکی از رایج</span><span dir="LTR">&lrm;</span><span lang="AR-SA">ترین انواع تومورهای عصبی مرکزی بشمار می</span><span lang="FA">&lrm;</span><span lang="AR-SA">رود. به&lrm; دلیل مقاومت تومور گلیوبلاستوم به شیمی</span><span lang="FA">&nbsp;د</span><span lang="AR-SA">رمانی و رادیوتراپی و عملی</span><span lang="FA">&lrm; ن</span><span lang="AR-SA">بودن برداشت کامل تومور به د</span><span lang="AR-SA">لیل ماهیت تهاجمی آن، یافتن درمان‌های جدید همواره مورد توجه است. مطالعات انجام&nbsp;</span><span lang="FA">&lrm;</span><span lang="AR-SA">شده در شرایط آزمایشگاهی نشان می‌دهد، پلاسمای سرد سلول ه</span><span lang="AR-SA">ای سرطانی را بدون آسیب</span><span lang="FA">&lrm;&nbsp;</span><span lang="AR-SA">رساندن به سلول</span><span lang="FA">&lrm; ه</span><span lang="AR-SA">ای طبیعی تخریب می</span><span lang="FA">&lrm;</span><span lang="AR-SA">کند. با توجه به ماهیت پیچیده پلاسما، اثرات بیولوژیکی آن به مشخصات منبع و نوع هدف تحت تیمار بستگی دارد. بنابراین در این مطالعه دو موضوع اساسی شامل بهینه&nbsp;</span><span lang="FA">&lrm;س</span><span lang="AR-SA">ازی ولتاژ، زمان و فاصله جت پلاسما برای تیمار رده سلول سرطان گلیوبلاستوما و اثر مخلوط 5 درصد گاز ملکولی اکسیژن با گاز کاری هلیوم و آرگون بر زنده‌مانی این سلول‌ها بررسی شد.</span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span class="StyleLatinHelveticaComplex2LotusLatin12ptComple" new="" roman="" times=""><span style="color:black"><b><span lang="AR-SA"><span style="color:red">مواد<span class="StyleLatinHelveticaComplex2LotusLatin12ptComple" new="" roman="" times=""><span style="color:black"><b> </b></span></span>و</span></span></b></span></span><span class="StyleLatinHelveticaComplex2LotusLatin12ptComple" new="" roman="" times=""><span style="color:black"><b> </b></span></span><span class="StyleLatinHelveticaComplex2LotusLatin12ptComple" new="" roman="" times=""><span style="color:black"><b><span lang="AR-SA"><span style="color:red">روش‌ها:</span></span></b></span></span> <span lang="AR-SA">رده</span> <span lang="AR-SA">سلولی</span> <span lang="AR-SA">سرطان</span> <span lang="AR-SA">گلیوبلاستوم</span> <span lang="AR-SA">انسانی</span> <span dir="LTR">U-87</span> <span dir="LTR">MG</span> <span lang="AR-SA">از</span> <span lang="AR-SA">بانک</span> <span lang="AR-SA">سلولی</span> <span lang="AR-SA">انستیتو</span> <span lang="AR-SA">پاستور</span> <span lang="AR-SA">ایران</span> <span lang="AR-SA">خریداری</span> <span lang="AR-SA">شد.</span> <span lang="AR-SA">به م</span><span lang="AR-SA">نظور</span> <span lang="AR-SA">بهینه س</span><span lang="AR-SA">ازی،</span> <span lang="AR-SA">5</span> <span lang="AR-SA">ولتاژ</span> <span lang="AR-SA">کاری،</span> <span lang="AR-SA">3</span> <span lang="AR-SA">فاصله</span> <span lang="AR-SA">نازل</span> <span lang="AR-SA">پلاسما</span> <span lang="AR-SA">تا</span> <span lang="AR-SA">سلول</span> <span lang="AR-SA">و</span> <span lang="AR-SA">یک</span> <span lang="AR-SA">مدت</span> <span lang="AR-SA">زمان</span> <span lang="AR-SA">تیمار</span> <span lang="AR-SA">در</span> <span lang="AR-SA">نظر</span> <span lang="AR-SA">گرفته</span> <span lang="AR-SA">شد</span> <span lang="AR-SA">و</span> <span lang="AR-SA">زنده‌مانی</span> <span lang="AR-SA">سلول‌های</span> <span lang="AR-SA">تیمار</span><span lang="FA">&lrm;</span><span lang="AR-SA">شده</span> <span lang="AR-SA">با</span> <span lang="AR-SA">تست</span> <span dir="LTR">MTT</span> <span lang="AR-SA">و</span> <span lang="AR-SA">براساس</span> <span lang="AR-SA">پروتکل</span> <span lang="AR-SA">سازنده</span> <span lang="AR-SA">اندازه‌گیری</span> <span lang="AR-SA">شد.</span> <span lang="AR-SA">پس</span> <span lang="AR-SA">از</span> <span lang="AR-SA">پیکربندی</span> <span lang="AR-SA">آزمایش</span> <span lang="AR-SA">در</span> <span lang="AR-SA">شرایط</span> <span lang="AR-SA">مطلوب،</span> <span lang="AR-SA">5</span> <span lang="AR-SA">مدت</span> <span lang="AR-SA">زمان</span> <span lang="AR-SA">تیمار</span> <span lang="AR-SA">مورد</span> <span lang="AR-SA">بررسی</span> <span lang="AR-SA">قرار</span> <span lang="AR-SA">گرفت.</span> <span lang="AR-SA">در</span> <span lang="AR-SA">آزمایش</span> <span lang="AR-SA">بررسی</span> <span lang="AR-SA">اثر</span> <span lang="AR-SA">نوع</span> <span lang="AR-SA">گاز</span> <span lang="AR-SA">کاری</span> <span lang="AR-SA">جت</span> <span lang="AR-SA">پلاسما</span> <span lang="AR-SA">بر</span> <span lang="AR-SA">زنده‌مانی</span> <span lang="AR-SA">سلول‌های</span> <span lang="AR-SA">سرطانی،</span> <span lang="AR-SA">5</span> <span lang="AR-SA">درصد</span> <span lang="AR-SA">گاز</span> <span lang="AR-SA">اکسیژن</span> <span lang="AR-SA">به</span> <span lang="AR-SA">گاز</span> <span lang="AR-SA">کاری</span> <span lang="AR-SA">هلیوم</span> <span lang="AR-SA">یا</span> <span lang="AR-SA">آرگون</span> <span lang="AR-SA">اضافه</span> <span lang="AR-SA">و</span> <span lang="AR-SA">نتایج</span> <span lang="AR-SA">مقایسه</span> <span lang="AR-SA">شد.</span> <span lang="AR-SA">تمام</span> <span lang="AR-SA">تجزیه</span> <span lang="AR-SA">و</span> <span lang="AR-SA">تحلیل‌های</span> <span lang="AR-SA">آماری</span> <span lang="AR-SA">با</span> <span lang="AR-SA">استفاده</span> <span lang="AR-SA">از</span> <span lang="AR-SA">نرم‌افزار</span> <span dir="LTR">SPSS</span> <span lang="AR-SA">و</span><span dir="LTR">Statistica</span> &nbsp;<span lang="AR-SA">انجام</span> <span lang="AR-SA">شد.</span> <span lang="AR-SA" style="font-family:IRLotus"></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span class="StyleLatinHelveticaComplex2LotusLatin12ptComple" new="" roman="" times=""><span style="color:black"><b><span lang="AR-SA"><span style="color:red">یافته‌ها:</span></span></b></span></span> <span lang="AR-SA">نتایج</span> <span lang="AR-SA">بهینه‌سازی</span> <span lang="AR-SA">ولتاژ</span> <span lang="AR-SA">و</span> <span lang="AR-SA">فاصله</span> <span lang="AR-SA">نشان</span> <span lang="AR-SA">داد</span> <span lang="AR-SA">که</span> <span lang="AR-SA">ولتاژ</span> <span lang="AR-SA">کاری</span> <span lang="AR-SA">5/4</span> <span lang="AR-SA">کیلو‌ولت</span> <span lang="AR-SA">و</span> <span lang="AR-SA">فاصله</span> <span lang="AR-SA">4</span> <span lang="AR-SA">سانتی‌متری</span> <span lang="AR-SA">اثر</span> <span lang="AR-SA">بهتری</span> <span lang="AR-SA">در</span> <span lang="AR-SA">مرگ</span> <span lang="FA">برنامه‌ریزی&lrm; ش</span><span lang="AR-SA">ده</span> <span lang="AR-SA">سلول‌های</span> <span dir="LTR">U-87</span> <span dir="LTR">MG</span> <span lang="AR-SA">برای</span> <span lang="AR-SA">پیکربندی</span> <span lang="AR-SA">جت</span> <span lang="AR-SA">پلاسمای</span> <span lang="AR-SA">انتخابی</span> <span lang="AR-SA">دارد.</span> <span lang="AR-SA">علاوه&nbsp;</span><span lang="FA">&lrm;ب</span><span lang="AR-SA">راین،</span> <span lang="AR-SA">نتایج</span> <span lang="AR-SA">وابستگی</span> <span lang="AR-SA">معکوس</span> <span lang="AR-SA">زمان</span> <span lang="AR-SA">تیمار</span> <span lang="AR-SA">و</span> <span lang="AR-SA">زنده‌مانی</span> <span lang="AR-SA">سلول‌های</span> <span dir="LTR">U-87</span> <span dir="LTR">MG</span> <span lang="AR-SA">را</span> <span lang="AR-SA">نشان</span> <span lang="AR-SA">داد.</span> <span lang="AR-SA">ترکیب</span> <span lang="AR-SA">5</span> <span lang="AR-SA">درصد</span> <span lang="AR-SA">گاز</span> <span lang="AR-SA">ملکولی</span> <span lang="AR-SA">اکسیژن</span> <span lang="AR-SA">با</span> <span lang="AR-SA">هلیوم</span> <span lang="AR-SA">و</span> <span lang="AR-SA">آرگون</span> <span lang="AR-SA">منجر</span> <span lang="AR-SA">به</span> <span lang="AR-SA">افزایش</span> <span lang="AR-SA">زنده‌مانی</span> <span lang="AR-SA">و</span> <span lang="AR-SA">درنتیجه</span> <span lang="AR-SA">کاهش</span> <span lang="AR-SA">بازده</span> <span lang="AR-SA">اثربخشی</span> <span lang="AR-SA">پلاسما</span> <span lang="AR-SA">شد.</span></span></span><br> <span class="StyleLatinHelveticaComplex2LotusLatin12ptComple" new="" roman="" times=""><span style="color:black"><b><span dir="RTL" lang="AR-SA"><span style="color:red">نتیجه‌گیری:</span></span></b></span></span><span dir="RTL" lang="AR-SA"> به &lrm;طور</span><span dir="RTL" lang="FA">&lrm;</span><span dir="RTL" lang="AR-SA">کلی مطالعه حاضر نشان داد پارامترهای مختلف جت پلاسما شامل ولتاژ، مدت زمان تیمار، فاصله و نوع گاز کاری بر زنده‌مانی سلول‌های </span>U-87 MG اثر گذار است و شرایط بهینه تیمار هر رده سلولی با توجه به نوع دستگاه اختصاصی می‌باشد</span></span></span></div> <span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">Background</span></span></span></span></i></b><b><i> </i></b><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">and</span></span></span></span></i></b><b><i> </i></b><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">purpose:</span></span></span></span></i></b> <span new="" roman="" style="font-family:" times="">Brain</span> <span new="" roman="" style="font-family:" times="">tumors</span> <span new="" roman="" style="font-family:" times="">are</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">consequence</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">abnormal</span> <span new="" roman="" style="font-family:" times="">growth</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">cells,</span> <span new="" roman="" style="font-family:" times="">which</span> <span new="" roman="" style="font-family:" times="">may</span> <span new="" roman="" style="font-family:" times="">be</span> <span new="" roman="" style="font-family:" times="">benign</span> <span new="" roman="" style="font-family:" times="">or</span> <span new="" roman="" style="font-family:" times="">malignant</span> <span new="" roman="" style="font-family:" times="">depending</span> <span new="" roman="" style="font-family:" times="">on</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">nature</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">constituent</span> <span new="" roman="" style="font-family:" times="">cells.</span> <span new="" roman="" style="font-family:" times="">Glioblastoma</span> <span new="" roman="" style="font-family:" times="">multiforme</span> <span new="" roman="" style="font-family:" times="">(GBM)</span> <span new="" roman="" style="font-family:" times="">is</span> <span new="" roman="" style="font-family:" times="">one</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">most</span> <span new="" roman="" style="font-family:" times="">prevalent</span> <span new="" roman="" style="font-family:" times="">types</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">central</span> <span new="" roman="" style="font-family:" times="">nervous</span> <span new="" roman="" style="font-family:" times="">system</span> <span new="" roman="" style="font-family:" times="">tumors.</span> <span new="" roman="" style="font-family:" times="">Due</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">resistance</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">glioblastoma</span> <span new="" roman="" style="font-family:" times="">tumor</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">chemotherapy</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">radiotherapy</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">impracticality</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">complete</span> <span new="" roman="" style="font-family:" times="">tumor</span> <span new="" roman="" style="font-family:" times="">removal</span> <span new="" roman="" style="font-family:" times="">because</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">its</span> <span new="" roman="" style="font-family:" times="">aggressive</span> <span new="" roman="" style="font-family:" times="">nature,</span> <span new="" roman="" style="font-family:" times="">finding</span> <span new="" roman="" style="font-family:" times="">a</span> <span new="" roman="" style="font-family:" times="">novel</span> <span new="" roman="" style="font-family:" times="">approach</span> <span new="" roman="" style="font-family:" times="">is</span> <span new="" roman="" style="font-family:" times="">always</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">great</span> <span new="" roman="" style="font-family:" times="">interest.</span> <span new="" roman="" style="font-family:" times="">Studies</span> <span new="" roman="" style="font-family:" times="">conducted</span> <span new="" roman="" style="font-family:" times="">under</span> <span new="" roman="" style="font-family:" times="">laboratory</span> <span new="" roman="" style="font-family:" times="">conditions</span> <span new="" roman="" style="font-family:" times="">indicate</span> <span new="" roman="" style="font-family:" times="">that</span> <span new="" roman="" style="font-family:" times="">cold</span> <span new="" roman="" style="font-family:" times="">plasma</span> <span new="" roman="" style="font-family:" times="">destroys</span> <span new="" roman="" style="font-family:" times="">cancer</span> <span new="" roman="" style="font-family:" times="">cells</span> <span new="" roman="" style="font-family:" times="">without</span> <span new="" roman="" style="font-family:" times="">harming</span> <span new="" roman="" style="font-family:" times="">normal</span> <span new="" roman="" style="font-family:" times="">cells.</span> <span new="" roman="" style="font-family:" times="">Owing</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">complex</span> <span new="" roman="" style="font-family:" times="">nature</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">plasma,</span> <span new="" roman="" style="font-family:" times="">its</span> <span new="" roman="" style="font-family:" times="">biological</span> <span new="" roman="" style="font-family:" times="">effects</span> <span new="" roman="" style="font-family:" times="">rest</span> <span new="" roman="" style="font-family:" times="">on</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">characteristics</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">source</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">type</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">target</span> <span new="" roman="" style="font-family:" times="">being</span> <span new="" roman="" style="font-family:" times="">treated.</span> <span new="" roman="" style="font-family:" times="">Therefore,</span> <span new="" roman="" style="font-family:" times="">in</span> <span new="" roman="" style="font-family:" times="">this</span> <span new="" roman="" style="font-family:" times="">study,</span> <span new="" roman="" style="font-family:" times="">two</span> <span new="" roman="" style="font-family:" times="">basic</span> <span new="" roman="" style="font-family:" times="">issues</span> <span new="" roman="" style="font-family:" times="">including</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">optimization</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">plasma</span> <span new="" roman="" style="font-family:" times="">jet</span> <span new="" roman="" style="font-family:" times="">voltage,</span> <span new="" roman="" style="font-family:" times="">time</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">distance</span> <span new="" roman="" style="font-family:" times="">for</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">treatment</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">glioblastoma</span> <span new="" roman="" style="font-family:" times="">cancer</span> <span new="" roman="" style="font-family:" times="">cell</span> <span new="" roman="" style="font-family:" times="">line</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">effect</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">a</span> <span new="" roman="" style="font-family:" times="">mixture</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">5%</span> <span new="" roman="" style="font-family:" times="">molecular</span> <span new="" roman="" style="font-family:" times="">oxygen</span> <span new="" roman="" style="font-family:" times="">gas</span> <span new="" roman="" style="font-family:" times="">with</span> <span new="" roman="" style="font-family:" times="">helium</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">argon</span> <span new="" roman="" style="font-family:" times="">working</span> <span new="" roman="" style="font-family:" times="">gases</span> <span new="" roman="" style="font-family:" times="">on</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">viability</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">these</span> <span new="" roman="" style="font-family:" times="">cells</span> <span new="" roman="" style="font-family:" times="">were</span> <span new="" roman="" style="font-family:" times="">investigated.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">Materials</span></span></span></span></i></b><b><i> </i></b><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">and</span></span></span></span></i></b><b><i> </i></b><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">methods:</span></span></span></span></i></b> <span new="" roman="" style="font-family:" times="">Human</span> <span new="" roman="" style="font-family:" times="">glioblastoma</span> <span new="" roman="" style="font-family:" times="">cancer</span> <span new="" roman="" style="font-family:" times="">cell</span> <span new="" roman="" style="font-family:" times="">line</span> <span new="" roman="" style="font-family:" times="">U-87</span> <span new="" roman="" style="font-family:" times="">MG</span> <span new="" roman="" style="font-family:" times="">was</span> <span new="" roman="" style="font-family:" times="">purchased</span> <span new="" roman="" style="font-family:" times="">from</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">cell</span> <span new="" roman="" style="font-family:" times="">bank</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">Pasteur</span> <span new="" roman="" style="font-family:" times="">Institute</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">Iran.</span> <span new="" roman="" style="font-family:" times="">For</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">optimization</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">occur,</span> <span new="" roman="" style="font-family:" times="">five</span> <span new="" roman="" style="font-family:" times="">working</span> <span new="" roman="" style="font-family:" times="">voltages,</span> <span new="" roman="" style="font-family:" times="">three</span> <span new="" roman="" style="font-family:" times="">plasma</span> <span new="" roman="" style="font-family:" times="">nozzle-to-cell</span> <span new="" roman="" style="font-family:" times="">distances</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">a</span> <span new="" roman="" style="font-family:" times="">treatment</span> <span new="" roman="" style="font-family:" times="">duration</span> <span new="" roman="" style="font-family:" times="">were</span> <span new="" roman="" style="font-family:" times="">considered,</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">viability</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">treated</span> <span new="" roman="" style="font-family:" times="">cells</span> <span new="" roman="" style="font-family:" times="">was</span> <span new="" roman="" style="font-family:" times="">measured</span> <span new="" roman="" style="font-family:" times="">by</span> <span new="" roman="" style="font-family:" times="">MTT</span> <span new="" roman="" style="font-family:" times="">test</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">based</span> <span new="" roman="" style="font-family:" times="">on</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">manufacturer&#39;s</span> <span new="" roman="" style="font-family:" times="">protocol.</span> <span new="" roman="" style="font-family:" times="">After</span> <span new="" roman="" style="font-family:" times="">configuring</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">experiment</span> <span new="" roman="" style="font-family:" times="">under</span> <span new="" roman="" style="font-family:" times="">optimal</span> <span new="" roman="" style="font-family:" times="">conditions,</span> <span new="" roman="" style="font-family:" times="">five</span> <span new="" roman="" style="font-family:" times="">treatment</span> <span new="" roman="" style="font-family:" times="">durations</span> <span new="" roman="" style="font-family:" times="">were</span> <span new="" roman="" style="font-family:" times="">investigated.</span> <span new="" roman="" style="font-family:" times="">In</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">experiment</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">investigate</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">effect</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">plasma</span> <span new="" roman="" style="font-family:" times="">jet</span> <span new="" roman="" style="font-family:" times="">working</span> <span new="" roman="" style="font-family:" times="">gas</span> <span new="" roman="" style="font-family:" times="">on</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">viability</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">cancer</span> <span new="" roman="" style="font-family:" times="">cells,</span> <span new="" roman="" style="font-family:" times="">5%</span> <span new="" roman="" style="font-family:" times="">oxygen</span> <span new="" roman="" style="font-family:" times="">gas</span> <span new="" roman="" style="font-family:" times="">was</span> <span new="" roman="" style="font-family:" times="">added</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">helium</span> <span new="" roman="" style="font-family:" times="">or</span> <span new="" roman="" style="font-family:" times="">argon</span> <span new="" roman="" style="font-family:" times="">working</span> <span new="" roman="" style="font-family:" times="">gas</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">results</span> <span new="" roman="" style="font-family:" times="">were</span> <span new="" roman="" style="font-family:" times="">compared.</span> <span new="" roman="" style="font-family:" times="">All</span> <span new="" roman="" style="font-family:" times="">statistical</span> <span new="" roman="" style="font-family:" times="">analyses</span> <span new="" roman="" style="font-family:" times="">were</span> <span new="" roman="" style="font-family:" times="">performed</span> <span new="" roman="" style="font-family:" times="">using</span> <span new="" roman="" style="font-family:" times="">SPSS</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">Statistica</span> <span new="" roman="" style="font-family:" times="">softwares.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">Results:</span></span></span></span></i></b><span new="" roman="" style="font-family:" times=""> The results of voltage and distance optimization demonstrated that the working voltage of 4.5 kV and 4 cm distance proves to be more effective on the programmed death of U-87 MG cells for the selected plasma jet configuration. In addition, the results showed the inverse dependence of treatment time and survival of U-87 MG cells. The combination of 5% oxygen molecular gas with helium and argon led to an increase in survival rate and as a result, a decrease in plasma effectiveness.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:90%"><span style="font-family:Calibri,sans-serif"><b><i><span style="font-size:10.5pt"><span style="line-height:90%"><span new="" roman="" style="font-family:" times=""><span style="color:red">Conclusion:</span></span></span></span></i></b> <span new="" roman="" style="font-family:" times="">In</span> <span new="" roman="" style="font-family:" times="">general,</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">present</span> <span new="" roman="" style="font-family:" times="">study</span> <span new="" roman="" style="font-family:" times="">indicated</span> <span new="" roman="" style="font-family:" times="">that</span> <span new="" roman="" style="font-family:" times="">different</span> <span new="" roman="" style="font-family:" times="">parameters</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">plasma</span> <span new="" roman="" style="font-family:" times="">jet,</span> <span new="" roman="" style="font-family:" times="">including</span> <span new="" roman="" style="font-family:" times="">voltage,</span> <span new="" roman="" style="font-family:" times="">duration</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">treatment,</span> <span new="" roman="" style="font-family:" times="">distance</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">type</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">working</span> <span new="" roman="" style="font-family:" times="">gas</span> <span new="" roman="" style="font-family:" times="">have</span> <span new="" roman="" style="font-family:" times="">an</span> <span new="" roman="" style="font-family:" times="">effect</span> <span new="" roman="" style="font-family:" times="">on</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">viability</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">U-87</span> <span new="" roman="" style="font-family:" times="">MG</span> <span new="" roman="" style="font-family:" times="">cells,</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">optimal</span> <span new="" roman="" style="font-family:" times="">treatment</span> <span new="" roman="" style="font-family:" times="">conditions</span> <span new="" roman="" style="font-family:" times="">for</span> <span new="" roman="" style="font-family:" times="">each</span> <span new="" roman="" style="font-family:" times="">cell</span> <span new="" roman="" style="font-family:" times="">line</span> <span new="" roman="" style="font-family:" times="">are</span> <span new="" roman="" style="font-family:" times="">specific</span> <span new="" roman="" style="font-family:" times="">according</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">type</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">device.</span></span></span></span><br> &nbsp; پلاسمای سرد؛ سرطان گلیوبلاستوما؛ بهینه‌سازی, پارامترهای پلاسما Cold plasma, Glioblastoma cancer, Optimization of plasma parameters 22 30 http://icml.ir/browse.php?a_code=A-10-548-2&slc_lang=fa&sid=1 Mahdieh Bakhtiari مهدیه بختیاری رمضانی mahdiyeh.bakhtiyari@gmail.com 1050031947532846004601 1050031947532846004601 Yes عضو هیئت علمی پژوهشکده پلاسما و گداخت هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، تهران، ایران Aboturab NaimAbadi ابوتراب نعیم آبادی 1050031947532846004602 1050031947532846004602 No عضو هیئت علمی پژوهشکده پلاسما و گداخت هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، تهران، ایران Fatemeh Shakeri فاطمه شاکری 1050031947532846004603 1050031947532846004603 No عضو هیئت علمی پژوهشکده پلاسما و گداخت هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، تهران، ایران