fa بررسی تأثیر استفاده از پلیمر نیمه‎ طبیعی HPMC در شفاف‎سازی پوست موش عمومى General پژوهشي Research <div style="text-align: justify;"><span style="line-height:2;"><span style="font-family:Tahoma;"><span style="font-size:12px;"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="AR-SA"><span style="color:red">مقدمه:</span> امروزه شفاف</span><span lang="FA">&nbsp;س</span><span lang="AR-SA">ازی بر روی بافت</span><span lang="FA">&lrm; ه</span><span lang="AR-SA">ای زیادی بخصوص پوست صورت بالینی و آزمایشگاهی می</span><span lang="FA">&lrm;</span><span lang="AR-SA">گیرد. مواد تشکیل&nbsp;</span><span lang="FA">&lrm;</span><span lang="AR-SA">دهنده بافت که عمدتاً پروتئین ه</span><span lang="AR-SA">ا، لیپید</span><span lang="FA">&lrm;</span><span lang="AR-SA">ها، آب میان بافتی و عروق خونی است، باعث پراکندگی شدید نور شده و نفوذ نور به اعماق بافت را محدود می</span><span lang="FA">&lrm;</span><span lang="AR-SA">کنند. این محدودیت باعث عدم دستیابی به اطلاعات ساختاری و تشکیل&nbsp;</span><span lang="AR-SA">دهنده بافت توسط نور و کاهش در وضوح تصویربرداری نوری می</span><span lang="FA">&lrm;</span><span lang="AR-SA">شود. در این مطالعه با استفاده از شفاف&nbsp;</span><span lang="FA">&lrm;س</span><span lang="AR-SA">ازهای مبتنی بر پلیمر نیمه&nbsp;</span><span lang="FA">&lrm;</span><span lang="AR-SA">طبیعی </span><span dir="LTR">HPMC</span><span lang="AR-SA">&nbsp; و سوربیتول و با بکارگیری روغن نارگیل، توئین 20 (که به اختصار </span><span dir="LTR">CSC1</span><span lang="AR-SA"> و </span><span dir="LTR">CSC2</span><span lang="AR-SA"> نامیده شدند)، پارامتر</span><span lang="FA">&lrm;</span><span lang="AR-SA">های اپتیکی نمونه بافت پوست توسط روش طیف نشر بازتابی (</span><span dir="LTR">DRS</span><span lang="AR-SA">) مورد بررسی قرار گرفت. </span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="AR-SA"><span style="color:red">مواد و روش ه</span></span><span lang="FA"><span style="color:red">ا</span></span><span lang="AR-SA"><span style="color:red">:</span> برای بدست&nbsp;</span><span lang="FA">&lrm;</span><span lang="AR-SA">آوردن طیف ه</span><span lang="AR-SA">ای بازتابی و عبوری بافت از روش طیف </span><span lang="AR-SA">سنجی نشر بازتابی (</span><span dir="LTR">DRS</span><span lang="AR-SA">) استفاده شده است. سپس با استفاده از مقادیر بدست آ</span><span lang="AR-SA">مده و جایگذاری در رابطه کرامرز -کرونیگ می</span><span lang="FA">&lrm;</span><span lang="AR-SA">توان ضریب شکست را قبل و بعد از اعمال شفاف س</span><span lang="AR-SA">از بدست آورد. همچنین پارامتر</span><span lang="FA">&lrm;</span><span lang="AR-SA">های اپتیکی دیگر نظیر ضریب جذب، ضریب تضعیف کل و ضریب انتشار از طیف ه</span><span lang="AR-SA">ای بازتابی و عبوری محاسبه شده است.</span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="AR-SA"><span style="color:red">یافته‌ها:</span> تاثیر استفاده از دو محلول پیشنهادی روی</span> <span lang="AR-SA">تغییرات ضریب شکست، ضریب جذب و ضریب انتشار محلول ه</span><span lang="AR-SA">ای شفاف</span><span lang="FA">&lrm;</span><span lang="AR-SA">ساز در فرایند شفاف</span><span lang="FA">&lrm;</span><span lang="AR-SA">سازی پوست موش بررسی شد.</span><span lang="FA"><span style="font-family:IRLotus"></span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="AR-SA"><span style="color:red">نتیجه‌گیری:</span> می</span><span lang="FA">&lrm;</span><span lang="AR-SA">توان گفت که پلیمر نیمه&nbsp;</span><span lang="FA">&lrm;ط</span><span lang="AR-SA">بیعی </span><span dir="LTR">HPMC</span><span lang="AR-SA"> در حفظ ساختار محلول شفاف</span><span lang="FA">&lrm;</span><span lang="AR-SA">ساز نقش موثری داشته است. از این رو می</span><span lang="FA">&lrm;</span><span lang="AR-SA">توان نتیجه گرفت که محلول </span><span dir="LTR">CSC2</span><span lang="AR-SA"> شفاف س</span><span lang="AR-SA">ازی را بهتر انجام داده و در</span><span lang="FA">&lrm;</span><span lang="AR-SA">نهایت نور بیشتر به بافت نفوذ خواهد کرد.</span></span></span></span></span></span><span style="font-family:Tahoma;"><span style="font-size:12px;"><span style="line-height:95%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="FA" style="line-height:95%"><span style="font-family:IRLotus"></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="line-height:2;"><span style="font-family:Tahoma;"><span style="font-size:12px;">&nbsp;</span></span></span></div> <span style="font-size:11pt"><span style="line-height:95%"><span style="font-family:Calibri,sans-serif"><b><i><span new="" roman="" style="font-family:" times=""><span style="color:red">Introduction:</span></span></i></b><span new="" roman="" style="font-family:" times=""> The</span> <span new="" roman="" style="font-family:" times="">skin</span> <span new="" roman="" style="font-family:" times="">is</span> <span new="" roman="" style="font-family:" times="">considered</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">first</span> <span new="" roman="" style="font-family:" times="">barrier</span> <span new="" roman="" style="font-family:" times="">against</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">penetration</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">laser</span> <span new="" roman="" style="font-family:" times="">light</span> <span new="" roman="" style="font-family:" times="">into</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">body,</span> <span new="" roman="" style="font-family:" times="">which</span> <span new="" roman="" style="font-family:" times="">causes</span> <span new="" roman="" style="font-family:" times="">limitations</span> <span new="" roman="" style="font-family:" times="">in</span> <span new="" roman="" style="font-family:" times="">imaging</span> <span new="" roman="" style="font-family:" times="">or</span> <span new="" roman="" style="font-family:" times="">treatment</span> <span new="" roman="" style="font-family:" times="">with</span> <span new="" roman="" style="font-family:" times="">laser</span> <span new="" roman="" style="font-family:" times="">light.</span> <span new="" roman="" style="font-family:" times="">The</span> <span new="" roman="" style="font-family:" times="">constituents</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">skin,</span> <span new="" roman="" style="font-family:" times="">which</span> <span new="" roman="" style="font-family:" times="">are</span> <span new="" roman="" style="font-family:" times="">mainly</span> <span new="" roman="" style="font-family:" times="">proteins,</span> <span new="" roman="" style="font-family:" times="">lipids,</span> <span new="" roman="" style="font-family:" times="">interstitial</span> <span new="" roman="" style="font-family:" times="">water,</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">blood</span> <span new="" roman="" style="font-family:" times="">vessels,</span> <span new="" roman="" style="font-family:" times="">cause</span> <span new="" roman="" style="font-family:" times="">severe</span> <span new="" roman="" style="font-family:" times="">light</span> <span new="" roman="" style="font-family:" times="">scattering</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">limit</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">penetration</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">light</span> <span new="" roman="" style="font-family:" times="">into</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">deep</span> <span new="" roman="" style="font-family:" times="">tissues.</span> <span new="" roman="" style="font-family:" times="">This</span> <span new="" roman="" style="font-family:" times="">limitation</span> <span new="" roman="" style="font-family:" times="">causes</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">lack</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">access</span> <span new="" roman="" style="font-family:" times="">to</span> <span new="" roman="" style="font-family:" times="">structural</span> <span new="" roman="" style="font-family:" times="">information</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">tissue</span> <span new="" roman="" style="font-family:" times="">constituents</span> <span new="" roman="" style="font-family:" times="">by</span> <span new="" roman="" style="font-family:" times="">light</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">a</span> <span new="" roman="" style="font-family:" times="">decrease</span> <span new="" roman="" style="font-family:" times="">in</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">resolution</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">optical</span> <span new="" roman="" style="font-family:" times="">imaging.</span> <span new="" roman="" style="font-family:" times="">In</span> <span new="" roman="" style="font-family:" times="">this</span> <span new="" roman="" style="font-family:" times="">study,</span> <span new="" roman="" style="font-family:" times="">using</span> <span new="" roman="" style="font-family:" times="">semi-natural</span> <span new="" roman="" style="font-family:" times="">HPMC</span> <span new="" roman="" style="font-family:" times="">polymer,</span> <span new="" roman="" style="font-family:" times="">sorbitol,</span> <span new="" roman="" style="font-family:" times="">coconut</span> <span new="" roman="" style="font-family:" times="">oil</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">Tween</span> <span new="" roman="" style="font-family:" times="">20</span> <span new="" roman="" style="font-family:" times="">(called</span> <span new="" roman="" style="font-family:" times="">CSC1</span> <span new="" roman="" style="font-family:" times="">and</span> <span new="" roman="" style="font-family:" times="">CSC2</span> <span new="" roman="" style="font-family:" times="">for</span> <span new="" roman="" style="font-family:" times="">short),</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">optical</span> <span new="" roman="" style="font-family:" times="">parameters</span> <span new="" roman="" style="font-family:" times="">of</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">skin</span> <span new="" roman="" style="font-family:" times="">tissue</span> <span new="" roman="" style="font-family:" times="">sample</span> <span new="" roman="" style="font-family:" times="">were</span> <span new="" roman="" style="font-family:" times="">determined</span> <span new="" roman="" style="font-family:" times="">by</span> <span new="" roman="" style="font-family:" times="">the</span> <span new="" roman="" style="font-family:" times="">diffuse</span> <span new="" roman="" style="font-family:" times="">reflection</span> <span new="" roman="" style="font-family:" times="">spectrum</span> <span new="" roman="" style="font-family:" times="">(DRS)</span> <span new="" roman="" style="font-family:" times="">method.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:95%"><span style="font-family:Calibri,sans-serif"><b><i><span new="" roman="" style="font-family:" times=""><span style="color:red">Research method:</span></span></i></b><span new="" roman="" style="font-family:" times=""> DRS was used to obtain the reflection and transmission spectra of the tissue. Then, by using the obtained values and placing them in the Kramers-Kronig relationship, the refractive index can be obtained before and after applying optical clearing agent. Also, other optical parameters such as absorption coefficient, total attenuation coefficient and diffusion coefficient have been calculated from reflection and transmission spectra.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:95%"><span style="font-family:Calibri,sans-serif"><b><i><span new="" roman="" style="font-family:" times=""><span style="color:red">Findings:</span></span></i></b><span new="" roman="" style="font-family:" times=""> The effect of using optical clearing agent (CSC1, CSC2) on variation of optical properties of sample (refractive index, absorption coefficient, and diffusion coefficient) are measured. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:95%"><span style="font-family:Calibri,sans-serif"><b><i><span new="" roman="" style="font-family:" times=""><span style="color:red">Conclusion:</span></span></i></b><span new="" roman="" style="font-family:" times=""> The results show that the semi-natural HPMC polymer, which played a role in maintaining the structure of the solution, was effective. Therefore, it can be concluded that the CSC2 could better clear the sample, and it improves the optical depth.</span></span></span></span><br> &nbsp; شفاف‎ساز اپتیکی, خواص اپتیکی پوست, کرامرز - کرونیگ Optical clearing agent, Optical properties of skin, Kramers-Kronig 5 12 http://icml.ir/browse.php?a_code=A-10-545-1&slc_lang=fa&sid=1 saeed ziaee سعید ضیائی 1050031947532846005099 1050031947532846005099 No گروه فوتونیک، آزمایشگاه تصویربرداری نوری زیستی، پژوهشکده لیزر و پلاسما، دانشگاه شهید‎بهشتی، mohammad Ali Ansari محمدعلی انصاری 1050031947532846005100 1050031947532846005100 Yes دانشجوی دکتری فوتونیک، گروه فوتونیک، آزمایشگاه تصویربرداری نوری زیستی، پژوهشکده لیزر و پلاسما، دانشگاه شهیدبهشتی، تهران، ایران Vahid Ghotbi Maleki وحید قطبی ملکی 1050031947532846005101 1050031947532846005101 No دانشجوی دکتری فیتوشیمی، آزمایشگاه فیتوشیمی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهیدبهشتی تهران Samad Nejad Ebrahimi صمد نژادابراهیمی 1050031947532846005102 1050031947532846005102 No دانشجوی دکتری فیتوشیمی، آزمایشگاه فیتوشیمی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهیدبهشتی تهران