fa مطالعه میزان تکثیر در رده سلولی بنیادی مزانشیمی تحت تاثیر کربوکسی تراپی و نور لیزر کم ‎توان عمومى General پژوهشي Research <div style="text-align: justify;"><span style="line-height:2;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="AR-SA"><span style="color:red">اهداف:</span></span></b> <span lang="AR-SA"><span style="color:black">با توجه به اهمیت روز افزون ترمیم و جوانسازی بافت</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">های مختلف لازم است توجه زیادی به تدوین پروتکل ه</span></span><span lang="AR-SA"><span style="color:black">ای درمانی جهت بهبود نتایج درمانی انجام شود. در حال حاضر از لیزر</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">های کم ت</span></span><span lang="AR-SA"><span style="color:black">وان در ترمیم بافت</span></span><span lang="FA"><span style="color:black">&lrm;&nbsp;</span></span><span lang="AR-SA"><span style="color:black">های پوستی استفاده می</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">شود. روش کربوکسی</span></span><span lang="FA"><span style="color:black">&lrm;&nbsp;</span></span><span lang="AR-SA"><span style="color:black">تراپی با توجه به درمان غیر</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">تهاجمی و ویژگی&nbsp;</span></span><span lang="AR-SA"><span style="color:black">های منحصر</span></span><span lang="FA"><span style="color:black">&lrm;&nbsp;</span></span><span lang="AR-SA"><span style="color:black">به ف</span></span><span lang="AR-SA"><span style="color:black">رد خود اهمیت ویژه</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">ای پیدا کرده است. در این روش بسته به ناحیه تحت درمان، مقداری دی ا</span></span><span lang="AR-SA"><span style="color:black">کسید</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">کربن به موضع&nbsp;تزریق&nbsp;می</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">شود. گرچه این ماده به صورت طبیعی توسط سلول ه</span></span><span lang="AR-SA"><span style="color:black">ای بدن تولید می</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">شود، اما زمانی که مقدار آن بیش&nbsp;</span></span><span lang="FA"><span style="color:black">&lrm;ا</span></span><span lang="AR-SA"><span style="color:black">ز</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">حد طبیعی شود؛ بدن را مجبور به پاسخ می</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">کند. زمانی که این ماده وارد بدن می</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">شود، سیستم گردش خون در ناحیه تحت درمان تحریک می</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">شود. بدن این شرایط جدید را به منزله کمبود اکسیژن در نظر گرفته و سعی می</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">کند با استفاده از رگ&nbsp;</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">های خونی، شرایط را کنترل کند. با توجه به اثرات بالینی لیزرهای کم ت</span></span><span lang="AR-SA"><span style="color:black">وان و همچنین روش کربوکسی ت</span></span><span lang="AR-SA"><span style="color:black">راپی لازم است اثرات کربوکسی و همچنین اثرات تواماً با لیزرهای کم ت</span></span><span lang="AR-SA"><span style="color:black">وان در سطح سلولی نیز بررسی شود. در این مطالعه میزان تکثیر و زنده&nbsp;</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">مانایی سلول ه</span></span><span lang="AR-SA"><span style="color:black">ای بنیادی مشتق از بافت چربی</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black"> تحت این تیمارها جهت کمک به تدوین پروتکل&nbsp;</span></span><span lang="FA"><span style="color:black">&lrm;ه</span></span><span lang="AR-SA"><span style="color:black">ای درمانی موثرتر بررسی شده است.</span></span></span></span></span></span></span><span style="line-height:1;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="direction:rtl"><span style="unicode-bidi:embed"><span dir="LTR"><span style="color:black"></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="line-height:2;"><span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="AR-SA"><span style="color:red">مواد و روش‌ها:</span></span></b><span lang="AR-SA"><span style="color:black"> مطالعه حاضر به صورت آزمایشگاهی بر روی سلول‌های بنیادی مشتق از بافت چربی انسانی انجام گردید. سلول </span></span><span dir="LTR"><span style="color:black">ADMSCs</span></span> <span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">از جهاد دانشگاهی مرکز ملی ذخایر ژنتیکی و زیستی ایران تهیه شد. کشت در محیط </span></span><span dir="LTR"><span style="color:black">DMEM [ + 10%</span></span> <span dir="LTR"><span style="color:black">FBS</span></span> <span lang="AR-SA"><span style="color:black">حاوی آنتی</span></span><span lang="FA"><span style="color:black">&lrm; ب</span></span><span lang="AR-SA"><span style="color:black">یوتیک یک درصد انجام گرفت. سلول</span></span><span lang="FA"><span style="color:black">&lrm; ه</span></span><span lang="AR-SA"><span style="color:black">ای بنیادی مشتق از چربی تحت تاثیر لیزر کم</span></span><span lang="FA"><span style="color:black">&lrm; ت</span></span><span lang="AR-SA"><span style="color:black">وان با دوز</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">های مختلف 3،2، 5 و 7 ژول بر سانتی م</span></span><span lang="AR-SA"><span style="color:black">تر</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">مربع و همچنین تحت تیمار</span></span><span lang="FA"><span style="color:black">&lrm; </span></span><span lang="AR-SA"><span style="color:black">کربوکسی با غلظت&nbsp;</span></span><span lang="AR-SA"><span style="color:black">های مختلف (با سرعت&nbsp;</span></span><span lang="FA"><span style="color:black">&lrm;ه</span></span><span lang="AR-SA"><span style="color:black">ا و حجم</span></span><span lang="FA"><span style="color:black">&lrm;&nbsp;</span></span><span lang="AR-SA"><span style="color:black">های مختلف) </span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">با دستگاه</span></span><span dir="LTR"><span style="color:black">Carboxy</span></span> <span dir="LTR"><span style="color:black">therapy </span></span>&nbsp;<span lang="AR-SA"><span style="color:black">در معرض گاز </span></span><span lang="AR-SA"><span style="color:black">CO2 </span></span><span lang="AR-SA"><span style="color:black">قرار داده شدند و سپس زنده&nbsp;</span></span><span lang="FA"><span style="color:black">&lrm;م</span></span><span lang="AR-SA"><span style="color:black">انایی سلولی توسط تست </span></span><span dir="LTR"><span style="color:black">MTT</span></span> <span lang="AR-SA"><span style="color:black">انجام شد.&nbsp; </span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="AR-SA"><span style="color:red">نتایج:</span></span></b><span lang="AR-SA"><span style="color:black"> نتایج حاصل از تست </span></span><span dir="LTR"><span style="color:black">MTT</span></span> <span lang="AR-SA"><span style="color:black">نشان د</span></span><span lang="AR-SA"><span style="color:black">هنده افزایش زنده&nbsp;</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">مانایی و تکثیر سلول</span></span><span lang="FA"><span style="color:black">&lrm;</span></span><span lang="AR-SA"><span style="color:black">ها تحت تیمارهای لیزر و کربوکسی بود و در تیمار همزمان لیزر و کربوکسی افزایش بیشتری را نسبت به گروه کنترل نشان داد. </span></span></span></span><br> <span style="direction:rtl"><span style="unicode-bidi:embed"><b><span lang="AR-SA"><span style="color:red">نتیجه‌گیری:</span></span></b><span lang="AR-SA"><span style="color:black"> براساس یافته</span></span><span lang="FA"><span style="color:black">&lrm; ه</span></span><span lang="AR-SA"><span style="color:black">ای مطالعه حاضر استفاده از کربوکسی</span></span><span lang="FA"><span style="color:black">&nbsp;ت</span></span><span lang="AR-SA"><span style="color:black">راپی و همچنین استفاده هم زمان لیزر با دوز بهینه می‌تواند به عنوان یک روش جایگزین در ترمیم پوست مورد استفاده قرار گیرد.</span></span></span></span><br> &nbsp;</span></span></span></div> <span style="font-size:12pt"><span style="line-height:87%"><span new="" roman="" style="font-family:" times=""><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">Due to the increasing importance of repairing and rejuvenating different tissues, it is necessary to pay attention to the development of treatment methods to improve treatment results. Low-level lasers are currently used to repair skin tissues. The carboxytherapy method has become particularly important due to its non-invasive treatment. In this method, some <a name="_Hlk155083989">carbon dioxide </a>is injected into the tissue.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:87%"><span new="" roman="" style="font-family:" times=""><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">It is produced naturally by the cells of the body, but when the amount of CO2 becomes too much, it forces the body to respond. When carbon dioxide enters the body, the circulatory system in the treated area is stimulated. And then tries to control the condition using blood vessels. Due to the clinical effects of low-level laser and also the carboxytherapy method, it is necessary to study carboxy effects and simultaneously the low-level laser at the cellular level. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:87%"><span new="" roman="" style="font-family:" times=""><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">In this study, the proliferation and viability of Adipose Mesenchymal Stem cells (MSC) have been studied under Laser and carboxy therapy treatments to help develop more effective protocols.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:87%"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:red">Materials</span></span></span></i></b><b><i> </i></b><b><i><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:red">and</span></span></span></i></b><b><i> </i></b><b><i><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:red">Methods:</span></span></span></i></b> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">Adipose</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">Mesenchymal</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">Stem</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">cells</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">were</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">cultured</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">in</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">DMEM</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">F12</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">+</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">10%</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">fetal</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">bovine</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">serum</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">(FBS),</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">100</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">U/ml</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">penicillin</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">and</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">100</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">mg/ml</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">streptomycin.</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">The</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">cells</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">were</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">placed</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">in</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">an</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">incubator</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">with</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">5%</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">CO2</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">humidified</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">atmosphere</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">at</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">37&deg;C.</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">MSC</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">stem</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">cells</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">were</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">irradiated</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">by</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">low-level</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">laser</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">with</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">various</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">doses</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">of</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">2,3,5</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">and</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">7</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">J/cm2</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">and</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">also</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">cells</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">were</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">exposed</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">to</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">carboxy</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">with</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">different</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">concentrations</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">(with</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">speeds</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">and</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">Different</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">volumes)</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">by</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">Carboxy</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">Therapy</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">device.</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">viability</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">of</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">cells</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">were</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">done</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">by</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">MTT</span></span></span> <span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525">test.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:87%"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:red">Results:</span></span></span></i></b><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525"> The results of this study illustrated the viability and proliferation of cells under the laser and carboxy therapy increased and at the simultaneous laser and carboxy treatment showed further increase than the control group.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:87%"><span new="" roman="" style="font-family:" times=""><b><i><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:red">Discussion:</span></span></span></i></b><span style="font-size:11.0pt"><span style="line-height:87%"><span style="color:#252525"> Based on the findings of this study, the use of carboxytherapy as well as the simultaneous use of laser with an optimal dose can be used as an alternative method in skin repair.</span></span></span></span></span></span><br> &nbsp; کربوکسی‎تراپی, زنده‎مانایی , لیزر کم‎توان, سلول‎های بنیادی مزانشیمی carboxy therapy, low level laser , Stem cells, Viability 13 21 http://icml.ir/browse.php?a_code=A-10-12-1&slc_lang=fa&sid=1 Mina Sadat Naderi میناسادات نادری 1050031947532846005217 1050031947532846005217 No گروه بیوفیزیک، دانشکده علوم زیستی، دانشگاه آزاد اسلامی واحد تهران شمال، ایران Seyed Mehdi Tabaie سیدمهدی طبایی Smtabaie@yahoo.com.ir 1050031947532846005216 1050031947532846005216 Yes گروه پژوهشی لیزر پزشکی، مرکز تحقیقات لیزر در پزشــکی، پژوهشــکده یارا Parnia Hemati پرنیا همتی 1050031947532846005218 1050031947532846005218 No گروه پژوهشی لیزر پزشکی، مرکز تحقیقات لیزر در پزشــکی، پژوهشــکده یارا، سازمان جهاد دانشگاهی علوم پزشکی تهران، ایران Afshan Shirkavand افشان شیرکوند 1050031947532846005219 1050031947532846005219 No گروه پژوهشی فتودینامیک تراپی، مرکز تحقیقات لیزر در پزشــکی، پژوهشــکده یارا، سازمان جهاد دانشگاهی علوم پزشکی تهران، ایران Mohammad hasan Soheilifar محمدحسن سهیلی فر 1050031947532846005220 1050031947532846005220 No گروه پژوهشی لیزر پزشکی، مرکز تحقیقات لیزر در پزشــکی، پژوهشــکده یارا، سازمان جهاد دانشگاهی علوم پزشکی تهران، ایران